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KMID : 0545120030130050710
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 5 p.710 ~ p.716
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Isolation of cDNAs for Gonadotropin-2 of Flounder (Paralichthys olivaceus) and Its Expressions in Adult Tissues
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LEE, JAE HYUNG
NAM, SOO WAN/KIM, YOUNG TAE
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Abstract
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Gonadotropin (GTH) is a pituitary glycoprotein hormone that regulates gonadal development in vertebrates. In teleosts, two types of gonadotropins, GTH-Ⅰ and GTH-Ⅱ,are produced in the pituitary, and they comprised of common α and distinct β subunits. In the present study, the cDNAs encoding GTH α and GTH-Ⅱβ subunits were cloned and sequenced from flounder (Paralichrhys olivuceus) pituitary cDNA library. The nucleotide sequence of the a subunit was 619 bp long, encoding 124 amino acids, and that of the GTH-Ⅱβ subunit was 538 bp long, encoding 145 amino acids. GTH subunits had well conserved cysteines, when aligned with other members of the glycoprotein family. The β subunit of gonadotropin I1 (GTH-IIP) had a difl´erent N-linked glycosylation site. RT-PCR analysis showed an increase of GTH I1 mRNA levels in association with gonadal development, and also showed that the mRNA expression of the a subunit was detected only in tissues from pituitary glands.
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